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Journal: International Journal of Molecular Sciences
Article Title: Glucosylceramide Synthase, a Key Enzyme in Sphingolipid Metabolism, Regulates Expression of Genes Accounting for Cancer Drug Resistance
doi: 10.3390/ijms26115112
Figure Lengend Snippet: GCS is involved in modulating gene expression of ovarian cancer cells in response to dactinomycin treatments. Cells of NCI/ADR-RES/Mock (Mock), NCI/ADR-RES/GCS (GCS), and NCI/ADR-RES/asGCS (asGCS) ovarian cancer cell lines were treated with 25 nM dactinomycin (DAC) in 5% FBS for 24 h. The mRNA levels of genes were analyzed by using Affymetrix GeneChip ® Human Genome U133 Plus 2.0 and Transcriptome Analysis Console (TAC 4.0). ( A ), differential gene-expression profiles of NCI/ADR-RES cells in response to DAC treatments. Differential expression of probed genes was identified as those having transcript levels 2-fold higher (up-regulated) or 2-fold lower (down-regulated) for the cells with treatment than those of comparator cells. ( B ), Overlaps of gene profiles of NCI/ADR-RES cells with treatments. GCS-responsive genes (overlap AB areas) are identified as those exhibiting their expression levels up-regulated in ADR-RES/GCS cells versus down-regulated in ADR/RES/asGCS cells (vice versa) treated with vehicle (left) or DAC (right), respectively.
Article Snippet: The
Techniques: Gene Expression, Quantitative Proteomics, Expressing
Journal: Biomedicines
Article Title: Integrative Multi-Omics Analysis Reveals Critical Molecular Networks Linking Intestinal-System Diseases to Colorectal Cancer Progression
doi: 10.3390/biomedicines12122656
Figure Lengend Snippet: Statistics of the DEGs for selected intestinal disorders and colon cancer.
Article Snippet: UC , GSE9686 , biopsy samples ,
Techniques: Control, Expressing, Next-Generation Sequencing